MachiBase Data Download
All the Drosophila melanogaster seven development stages transcription data are uploaded as follows.
- embryo.dat(tag length 36bp)
- larva.dat (tag length 36bp)
- oldFemale.dat (tag length 30bp)
- oldMale.dat (tag length 30bp)
- s2.dat (tag length 30bp)
- youngFemale.dat (tag length 30bp)
- youngMale.dat (tag length 30bp).
rawTag is archive for all Solexa tags as were collected. It includes
The mRNA products were digested with the EcoP15I type IIS restriction endonuclease. The digested 5'end mRNA tags are 25~27bp in length. The 5'end mRNA tags are processed with Solexa linker in a reaction mixture containing T4 DNA ligase in the supplied buffer. So each raw tags has Solexa linker after the 25th~27th bp. 26bpTagFasta is archive for all non-redundant tags truncated at 26bps. It has the following data files.
Multiply expressed mRNA 5'end Tags are summarized with their frequencies. The fasta sequence tags are named as tag_frequency.
ALL the transcription start site(TSS)s with their expression number determined by alinging 5'end tags are
listed in files inside the zip archive named. BLAT was used as alignment tool with three mismatches.